Abstracts - faqs.org

Abstracts

Biological sciences

Search abstracts:
Abstracts » Biological sciences

A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis

Article Abstract:

The randomly amplified polymorphic (RAPD) DNA fingerprinting technique has been used on a set of 101 strains of Bacillus genus, with 61 strains belonging to the B. cereus group. The purpose of the study was to discover an RAPD marker specific for the B. cereus group and for discriminating B. anthracis. The former causes food-borne disease syndromes while the latter is the active agent of the anthrax disease. The RAPD identified an 838-bp marker, the SG-850, for B. cereus, B. thuringiensis, B. anthracis and B. mycoides. It also has a 366-nucleotide open reading frame that is highly homologous to the ypuA gene of Bacillus subtilis.

Author: Borin, Sara, Daffonchio, Daniele, Fani, Renato, Gallo, Romina, Sorlini, Claudia, Frova, Giuseppe, Mori, Elena
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Bacterial genetics, Genetic markers

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA


Distribution of genes encoding putative virulence factors and fragment length polymorphisms in the vrrA gene among Brazilian isolates of Bacillus cereus and Bacillus thuringiensis

Article Abstract:

The distribution of genes encoding putative virulence factors, specifically, the Vip3a, BceT, PclA, Sph, NheB and NheC components of the nonhemolytic enterotoxin (NHE) complex and HblA of the hemolysin BL (HBL) complex, in a panel of Bacillus cereus and B. thuringiensis strains comprised mainly of Brazilian isolates are assessed. The variable region of the vrrA sequence for fragment length polymorphisms, which might prove useful for the specific identification of B. cereus and B. thuringiensis, are examined.

Author: Rabinovitch, Leon, Zahner, Viviane, Cabral, Diana Aparecida, Regua-Mangia, Adriana, Moreau, Gaetan, MaIntosh, Douglas
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA


Comparison of minisatellite polymorphisms in the Bacillus cereus complex: A simple assay for large-scale screening and identification of strains most closely related to Bacillus anthracis

Article Abstract:

Multiple-locus variable number of tandem repeat analysis (MLVA) is used to develop an assay for the identification of strains from the Bacillus cereus group most closely related to Bacillus anthracis. A very simple assay is proposed that allows the screening of hundreds of strains from the B. cereus complex, with modest equipment and at a low cost, to eventually fill the gap with B. anthracis and better understand the origin and making of this dangerous pathogen.

Author: Ramisse, Francoise, Hernandez, Eric, Valjevac, Samina, Hilaire, Valerie, Lisanti, Olivier, Vergnaud, Gilles, Pourcel, Christine, Cavallo, Jean-Didier
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Genetic research

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA


Subjects list: Research, Genetic aspects, Bacillus thuringiensis, Genetic polymorphisms, Bacillus cereus
Similar abstracts:
  • Abstracts: Discrimination of psychrotrophic and mesophilic strains of the Bacillus cereus group by PCR targeting of major cold shock protein genes
  • Abstracts: Amplified intergenic locus polymorphism as a basis for bacterial typing of Listeria spp. and Escherichia coli
  • Abstracts: Toxin-producing ability among Bacillus spp. outside the Bacillus cereus group. Differentiation of spores of Bacillus subtilis grown in different media by elemental characterization using time-of-flight secondary ion mass spectrometry
  • Abstracts: Paenibacillus sp. strain JDR-2 and XynA1: A novel system for methylglucuronoxylan utilization. Lactobacillus paracasei subsp. paracasei 8700:2 degrades insulin-type fructans exhibiting differen degrees of polymerization
  • Abstracts: Intra- and interspecific comparisons of bacterial diversity and community structure support coevolution of gut micorbiota and termite host
This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.
Some parts © 2023 Advameg, Inc.