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Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants

Article Abstract:

Saccharomyces cerevisiae SRS2 DNA helicase mutant alleles were found to vary in the degree of rad 18 suppression and hyperrecombination according to their location in the DNA helicase gene. The alleles were classified according to suppression of UV sensitivity of rad 18 mutants, reduction of meiotic viability, hyperrecombination and definition of the mutational change within the SRS2 gene. These alleles were obtained by the suppression of UV sensitivity of rad 18 mutants by srs2-101 mutants in the SRS2 gene.

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A colony color assay for Saccharomyces cerevisiae mutants defective in kinetochore structure and function

Article Abstract:

A study suggests the BLU1 gene may be necessary for maintaining optimum levels of kinetochore DNA-binding proteins in Saccharomyces cerevisiae (yeast). A colony color assay was used to observe kinetochore DNA-protein interactions in yeast. A lacZ reporter gene, controlled by a CEN-GAL1 promoter, was used to identify a blue color phenotype representing derepression of the promoter in colonies obtained from mutagenized cells. Three blu mutants, blu1, blu2 and blu3, were identified.

DNA binding proteins

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Possible cross-regulation of phosphate and sulfate metabolism in Saccharomyces cerevisiae

Article Abstract:

A study of plasmids responsible for suppressing methionine auxotrophy of cep1 null mutants of the CEP1 gene in Saccharomyces cerevisiae (yeast) suggests cross-regulation may exist between phosphate and sulfate metabolism. Subcloning experiments show the existence of correlation between an increase in PHO4 content of the plasmids and the degree of suppression.

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