Analysis of the effect exerted by extracellular pH on the maltose regulon in Escherichia coli K-12

Article Abstract:

The effects of the external pH (pHo) on the activities of the genetic promoters contained in the maltose regulon of the Escherichia coli were evaluated. The transport and catabolism of maltose and maltodextrins involved these genes belonging to five transcriptional units mediated by the transcriptional activator, the MalT protein. It was revealed that expression of the maltose regulon was mediated by pHo at the transcription level. The pH of the external medium affected malT transcription through the activity of the cAMP-cAMP receptor protein complex.

Hydrogen-ion concentration

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Escherichia coli RNase II: characterization of the promoters involved in the transcription of rnb

Article Abstract:

The rnb gene transcript is characterized in terms of its promoter and terminator regions. The rnb message, which is transcribed by two promoters, ends in a rho-independent terminator ten nucleotides downstream of rnb. The in vivo data provide strong evidence that the two promoters are really active. However, there is no in vitro transcription for the more efficient P2 promoter. This implies that an undetermined cis or trans factor is involved in transcription activation.

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The molecular basis for the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved activating region 1 contact of HlyX

Article Abstract:

A study was conducted on the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by fumarate and nitrate reduction (FNR). The findings indicate that hlyE is a Class I FNR-activated promoter. However, optimal positioning of the RNA polymerase to allow transcription is only made possible by HlyX. This further suggests that the AR1 of HlyX is superior to that of FNR.

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