Regulatory systems modulating the transcription of the pectinase gens of Erwinia chrysanthemi are conserved in Escherichia coli

Article Abstract:

Transcriptional fusions found on low-copy-number plasmids of the phytopathogenic enterobacterium Erwinia chrysanthemi were utilized to investigate the regulation of the pel genes in a heterologous host, Escherichia coli. The five pel fusions in E. coli were affected by growth phase, catabolite repression and anaerobic growth conditions and were induced in the presence of galacturonate, a sugar whose metabolism leads to the creation of KDG, the inducer of pel transcription in Er. chrysanthemi. Expression of pelE increased with the osmolarity of the culture medium. However, the regulation of pel expression by temperature or nitrogen starvation, seen in Er. chrysanthemi, was not seen in E. coli.

Genetic aspects, Metabolism

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Erwinia chrysanthemi at high osmolarity: influence of osmoprotectants on growth and pectate lyase production

Article Abstract:

An increase in the osmolarity of the culture medium decreases the growth and activity of the pectate lysases (PLs) activity of Erwinia chrysanthemi. In the presence of osmoprotectants such as glycine betaine, proline, ectoine and pipecolate the cell is able to grow in cultures containing high amounts of NaCl and the production of PL is unaffected by the increasing NaCl content. The uptake of the osmoprotectants and the activation of PL production because of induced transcription of the pelE gene is because of osmotic pressure but osmoprotectants have no effect on the PL activity in vitro.

Analysis, Fluid-electrolyte balance, Adaptation (Biology), Evolutionary adaptation, Osmoregulation

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Glutamine, glutamate, and alpha-glucosylglycerate are the major osmotic solutes accumulated by Erwinia chrysanthemi strain 3937

Article Abstract:

The Erwinia chrysanthemi 3937 endogenous osmolytes were analyzed and showed that the bacterium does not synthesize glutamine and alpha-glucosylglycerate in enterobacteria. The study showed that it is not true only halophilic organisms and its counterpart glucosylglycerol accumulate that glucosylglycerate by nonhalophilic ones.

Science & research, Bacteria, Glutamate, Glutamine, Chemical properties

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