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Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis

Article Abstract:

Bacillus subtilis produces surfactin, a cyclic lipopeptide antibiotic and biosurfactant, that increases the proliferation rates and changes the morphology of mycoplasma-contaminated mammalian cells. The high level of membrane phospholipids in the Mycoplasma cells makes the membranes susceptible to surfactin. Surfactin causes the disintegration of the mycoplasma membranes due to its physicochemical interaction with the outer part of the lipid membrane bilayer. The interaction makes the membranes permeable. Surfactin is active against Mycoplasma hyorhinis and M. orale.

Author: Pauli, Georg, Ozel, Muhsin, Vater, Joachim, Vollenbroich, Dirk
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Usage, Antibiotics, Physiological aspects, Bacillus subtilis, Mycoplasma, Surface active agents

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Culture variability associated with the U.S. Environmental Protection Agency Tuberculocidal Activity Test Method

Article Abstract:

Five Micobacterium bovis suspensions produced by laboratories using the Tuberculocidal Activity Test Method (TATM) exhibit significant difference between test cultures and culture-by-disinfectant interaction. The interaction effect signifies that the five cultures fail to respond in a consistent manner across different disinfectants. The results thus indicate that the culturing process fails to follow the standard procedure or to meet the test suspension criteria set by the Environmental Protection Agency's TATM.

Author: Carroll, Stephen J., Robison, Richard A., Leavitt, Ronald W., Osguthorpe, Russell J., Schaalje, G. Bruce, Ascenzi, Joseph M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
Analysis, Evaluation, United States. Environmental Protection Agency, Tuberculosis, Cultures (Biology)

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Modelling the growth kinetics of Phanerochaete chrysosporium in submerged static culture

Article Abstract:

The investigation and modelling of the growth kinetics of P. Chrysosporium ILNA-12 (CNCN I-398) under nonlimiting nitrogen and carbon conditions in submerged static culture is conducted. Nutrient limitation for induction lignin peroxidase is not required in this strain. A simple linear model, such as the Emerson model, Williams model or the Monod model, is capable of predicting biomass concentrations during the active growth phase.

Author: Legge, Raymond L., Barclay, Clayton D., Farquhar, Grahame F.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
Growth, Growth (Physiology)

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