Bacteriophage lambda as a cloning vector
Article Abstract:
A better understanding of lambda vectors for cloning has opened opportunities for investigators in the modification of the vector to suit the needs of their research. Due to the size of the lambda genome and its highly effective packaging system, it had been made to accomodate a wide array of inserts. Its central dispensable fragment can be replaced by a fragment of heterologous DNA to create replacement vectors. The use of antibody and nucleic acid probes has made screening of libraries constructruced in lambda vectors easy.
Publication Name: Microbiological Reviews
Subject: Biological sciences
ISSN: 0146-0749
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Ribosome regulation by the nascent peptide
Article Abstract:
The bacterial leader gene and the eukaryotic upstream open reading frame encode peptides that are cis-acting negative regulators of the translation of their ribosomes. The peptides possess less than 25 amino acids and are active before their emergence from the ribosomes. The nascent leader-encoded peptides select the site of ribosome stalling in leader mRNA by interfering with translation. The peptides are rRNA-binding peptides with targets at the peptidyl transferase centre of 23S rRNA.
Publication Name: Microbiological Reviews
Subject: Biological sciences
ISSN: 0146-0749
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Bacteriophage lysis: mechanism and regulation. Immune regulation in Epstein-Barr virus-associated diseases. Mechanism and regulation of eukaryotic protein synthesis
- Abstracts: Transcription termination signals in the nin region of bacteriophage lambda: identification of rho-dependent termination regions
- Abstracts: Mechanisms of directed mutation. Leber's hereditary optic neuropathy: the etiological role of a mutation in the mitochondrial cytochrome b gene