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Biological sciences

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Characterization of npf mutants identifying developmental genes in Physarum

Article Abstract:

The development of mutant Physarum polycephalum is caused by multiple pathways rather than a dependent sequence of events. Phenotypic analysis shows that different stages of plasmodium formation block different mutants and depend on the long cell cycle. Some of the mutations blocking apogamic development at an early stage failed to block sexual development in zygotes homozygous for the mutation. Mutations are more likely to occur when the development process is long and requires many changes to the cell structure.

Developmental genetics, Mutation (Biology), Mutation

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Molecular constituents of the replication apparatus in the plasmodium of Physarum polycephalum: identification by photoaffinity labelling

Article Abstract:

Research was conducted to examine the replication apparatus of Physarum polycephalum and to compare it with other systems of higher eukaryotes. The photoaffinity method was used to label replication protein A and enzymically active DNA polymerases in purified systems. Autoradiography and immunostaining were utilized for the identification of proteins. Results indicated the incorporation of AFBdCTP by P polycephalum DNA polymerase alpha in the dC position with lower frequencies in the dT, dA and dG positions.

Proteins, Protein crosslinking, Chromosome replication

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Contact with a solid substratum induces cysts in axenic cultures of Physarum polycephalum amoebae: mannitol-induced detergent-resistant cells are not true cysts

Article Abstract:

Cell type counts identified by transmission electron microscopy were compared with the frequencies of (triton)-resistant cells (TRC) in suspensions of amoeba from various treatments. Mannitol-treated TRC was found to be dense and rounded but without walls, while TRC formed after growth to stationary phase on bacterial lawns were walled. A solid substratum and nutrient deprivation was suggested to be required in efficient Plasmodium polycephalum development.

Genetic aspects, Composition, Amoeba, Amoebas, Cysts, Mannitol, Ultrastructure (Biology), Myxomycetes, Slime molds

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