Comparison of toxin overlay and solid-phase binding assays to identify diverse CryIA(c) toxin-binding proteins in Heliothis virescens midgut

Article Abstract:

The proteins of 170, 140, 120, 90, 75, 60 and 50 kDa, obtained from Heliothis virescens larval midguts, are linked to the radiolabeled CryIA(c) toxin, as detected from the solid-phase binding and the toxin overlay assays. The proteins are segregated in three toxin binding pools, by the anion-exchange fractionation. According to the toxin overlay assays, the protein sizes are the same for the pools but protein profiles are different. The solid-phase binding assay indicates that only one of three pools posses high-affinity binding proteins.

Research, Observations, Toxins, Heliothis, Corn earworm

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Predictive model for inactivation of feline calcicivirus, a norovirus surrogate, by heat and high hydrostatic pressure

Article Abstract:

A predictive model is described for studying the inactivation of a calicivirus by a combination of high hydrostatic pressure and temperature. The inactivation of feline calicivirus in a cell culture medium and still mineral water by high hydrostatic pressure and the heat is predicted by an approximated nth-order reaction model.

Physiological aspects, Environmental aspects, Chemical reaction, Rate of, Chemical kinetics, Hydrostatic pressure, Norovirus

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Molecular identification and genetic analysis of norovirus genogroups I and II in water environments: Comparative analysis of different reverse transcription-PCR assays

Article Abstract:

The analysis of environmental samples from seawater, estuarine water and effluents of sewage treatment plants for evaluating the role of environmental surface contamination as a possible vector for transmitting nerovirus genogroups I and II is discussed.

In-Vitro Diagnostic Substance Manufacturing, Diagnostic substances, Reverse Transcriptase, Sea-water, Seawater, Estuaries

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