Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor
Article Abstract:
The double coupling of glucose metabolism and citronellol esterification by acetyl coenzyme A (acetyl-CoA) in Hansenula saturnus cells present in an interface bioreactor produces citronellyl acetate. A high glucose content and citronellol addition increase accumulation of citronellyl acetate in the cells. The triple coupling of acetyl-CoA production, reduction of citronellal to citronellol and esterification of the citronellol by acetyl-CoA also produces citronellyl acetate. The coupling methods permit the formation of citronellyl acetate in the absence of acetyl donors.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Complete microbial degradation of both enantiomers of the chiral herbicide mecoprop ((RS)-2-(4-chloro-2-methylphenoxy)propionic acid) in an enantioselective manner by Sphingomonas herbicidovorans sp. nov.
Article Abstract:
Sphingomonas herbicidovorans MH utilizes the chiral herbicide mecoprop as a carbon and energy source, and completely degrades both R and S enantiomers of the herbicide in an enantioselective manner. The enantiomer-specific degradation pattern indicates that more than one enzyme system is involved in the degradation of the enantiomers. Stochiometric chloride release in growth experiments indicates degradation of the aromatic ring. The oxygen uptake rates by resting cell suspensions support the existence of enantioselective degradation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Autoradiographic method for isolation of diverse microbial species with unique catabolic traits
Article Abstract:
An autoradiographic method helps in the isolation of diverse microbial populations possessing unique catabolic traits without relying on selective enrichment techniques. The method involves the cultivation of bacteria through direct plating on microporous filters, incubation on radioactive films and autoradiography for identifying colonies possessing the desired catabolic phenotypes. The method is most helpful in isolating low frequency populations occurring in a background of populations without the catabolic phenotype of interest.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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