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Biological sciences

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Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria

Article Abstract:

A single, triplicate arbitrarily primed (AP)-PCR procedure for molecular fingerprinting of lactic acid bacteria has been developed. It is known that AP-PCR can be used to generate characteristic DNA fingerprint patterns but small changes in reaction conditions can bring band irreproducibility. One method that takes in triplicate reactions intentionally exposed to three annealing temperatures made bands reproducibly generated to be recognized possible. This was used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.

DNA, Identification and classification

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Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers

Article Abstract:

Researchers created species-specific PCR primers to distinguish several species of Lactobacillus using the recA gene sequence. These primers could identify individual species during a single reaction.

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Prophage-like elements in bifidobacteria: Insights from genomics, transcription, integration, distribution, and phylogenetic analysis

Article Abstract:

Three prophage-like elements those are present in the genomes of bifidobacterium breve UCC 2003 (Bbr-1), Bifidobacterium longum NCC 2705 (Bl-1), and Bifidobacterium longum DJO10A (Blj-1) are analyzed. The comparison of these prophage sequences with those available in the public databases shows that the bidifobacterial prophages possess a typical mosaic genome structure.

Science & research, Genetic aspects, Genomes, Bifidobacterium, Bifidobacteria

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