Dissecting the mechanisms of posttranscriptional gene silencing: divide and conquer
Article Abstract:
Two studies were able to dissect the mechanism of posttranscriptional gene silencing (PTGS) in transgenic plants into three phases; initiation, spread and maintenance. The study by Palauqui and Balzergue in 1999 was about the PTGS of a tobacco nitrate reductase cDNA, while the study by Voinnet et al. in 1998 examined PTGS through expression of the green fluorescent protein from the 35S promoter in Nicotiana benthamiana. Both studies confirmed the correctness of the ectopic pairing model by isolating the initiation of PTGS from is maintenance and spread.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Splitting hairs: dissecting roles of signaling systems in epidermal development
Article Abstract:
Developments in the understanding of hair follicle spacing and growth, including data on the effects of beta-catenin, are reviewed. Various experiments have shown that the mesoderm causes the epidermis to produce an evolutionary conserved signal that in turn induces the condensation of mesoderm cells to form the placode. After placode formation, one or more species-specific additional epithelial and mesoderm signals are believed to organize the specific skin structure.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing
Article Abstract:
Tobacco etch viruses encode P1/HC-Pro polyprotein that suppresses posttranscriptional gene silencing (PTGS). The PTGS, in turn, produces a number of aberrant RNAs in sequence-specific process in the cytoplasm. Experiments on Nicotiana tabacum plants show that transgenic lines loci with a highly expressed beta-glucuronidase contains PTGS. The results of genetic crosses, segregation analyses and nuclear transcriptional assays conducted on these plants are discussed.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Distinguishing the effects of mutational biases and natural selection on DNA sequence variation. Sodium orthovanadate-resistant mutants of Saccharomyces cerevisiae show defects in golgi-mediated protein glycosylation, sporulation and detergent resistance
- Abstracts: Simulation of effects of dominance on estimates of population genetic diversity and differentiation. RAPD genome maps of Douglas-fir
- Abstracts: Histone acetylation and transcriptional regulatory mechanisms. Nucleosome stability mediated by histone variants H3.3and H2A.Z
- Abstracts: Identification of a novel mechanism of regulation of Ah (dioxin) receptor function. Enhancer control of V(D)J recombination at the TCR-beta locus: differential effects on DNA cleavage and joining
- Abstracts: Identification of a novel mechanism of regulation of Ah (dioxin) receptor function. part 2 Gene targeting in the silkworm by use of a baculovirus