Effect of environmental factors on the trans/cis ratio of unsaturated fatty acids in Pseudomonas putida S12

Article Abstract:

The membrane cells of Pseudomonas putida S12 react to various environmental stress differently, showing varying trans/cis ratios of unsaturated fatty acids. An increase in the ratio of trans to cis characterizes the cell's reaction to the addition of heavy metals. Exposure at 30 degrees C results in optimal growth of cells, while gradual reduction in temperature decreases the saturation rate of fatty acids. Toxic concentrations of sodium chloride or sucrose increases the trans/cis ratio. Exposure to pH between 4.0 and 5.0 shows optimum cis/trans isomerase activity with a loss of viability.

Health aspects, Osmotic pressure, Unsaturated fatty acids, Hydrogen-ion concentration, Temperature, Heavy metals, Heavy metal poisoning

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Cell envelope changes in solvent-tolerant and solvent-sensitive Pseudomonas putida strains following exposure to o-xylene

Article Abstract:

A study was conducted to investigate the cell envelope dynamics of solvent-tolerant organisms. The phospholipid content, lipopolysaccharide structure and permeability of Pseudomonas putida Idaho and Pseudomonas putida MW1200 after exposure to o-Xylene were compared. Results showed that solvent tolerance occurs in the absence of metabolism of the organic solvent. Trans-unsaturated fatty acids were not found to confer solvent tolerance.

Cell research, Cytological research

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