Effects of carbon substrates on nitrite accumulation in freshwater sediments

Article Abstract:

The impact of several carbon compounds found in agricultural pollutants on nitrite accumulation in freshwater sediments has been studied. The carbon compounds were used as energy sources in 48-h incubation experiments to find out if the chemical structure of the pollutants influenced which NO(sub 3)(super -) reduction pathway was used and subsequent NO(sub 2)(super -) accumulation. Results reveal that the carbon stored within the sediments was sufficient to support DNRA and denitrifier populations. The NO(sub 2)(super -) concentration had a peak value of 80 micrograms of N per liter.

Environmental aspects, Carbon, Nitrites, Marine sediments, Agricultural pollution

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Bacterial viability and antibiotic susceptibility testing with SYTOX Green nucleic acid stain

Article Abstract:

A fluorescent nucleic acid stain that does not penetrate living cells was employed to evaluate the integrity of the plasma membranes of bacteria. SYTOX Green nucleic acid strain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. Binding of SYTOX Green stain to nucleic acids cause more than 500-fold improvement in fluorescence emission, causing bacteria with compromised plasma membranes to be brightly green fluorescent.

Cell membranes, Plasma membranes, Nucleic acids

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Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3

Article Abstract:

The conversion of aromatic hydrocarbons to aliphatic polyhydroxyalkanoate (PHA) is reported. PHA formation, the biochemical activity of whole cells, and the utilization of carbon and nitrogen throughout the growth cycle of Pseudomonas putida CA-3 with styrene, phenylacetic acid, and glucose as the sole sources of carbon and energy is also determined.

Pseudomonas putida, Polyhydroxyalkanoates, Phenylacetic acid

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