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Fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes to identify small phytoplankton by flow cytometry

Article Abstract:

A study to test the efficiency of in situ hybridization with rRNA fluorescent probes by flow cytometry for the detection of small photosynthetic eukaryotes shows that taxon-specific probes enhance the usage of flow cytometry for the identification of cultured pico and nonplanktonic strains deficient in taxonomically useful morphological characteristics. Analysis of the aligned sequence and the phylogeny of the 18S rRNA gene to detect the theoretical capacity of the probes to differentiate between distinct taxonomic groups of microalgae is the primary step during the test. Photosynthetic oceanic pico- and nonplanktonic eukaryotes are very useful in coastal waters.

Marine plankton

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Enumeration of marine viruses in culture and natural samples by flow cytometry

Article Abstract:

Flow cytometry (FCM) is compared with transmission electron microscopy and epifluorescence microscopy in its ability to detect marine viruses stained with the novel nucleic acid stain SYBR-I. The virus samples were treated with 0.5% glutaraldehyde and deep frozen for delayed analysis. The samples were then diluted in Tris-EDTA buffer and analyzed in the presence of SYBR-I. Results revealed that FCM can distinguish between two or sometimes three viral types quickly and accurately. This finding suggests FCM can be useful in studying viral infections in cultures.

Methods, Viruses, Identification and classification, Diagnostic virology

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A single species Micromonas pusilla (prasinophyceae), dominates the eukaryotic picoplankton in the western English Channel

Article Abstract:

The Prasinophyceae species comprises of various photosynthetic picoueukaryotic species, which are illustrated from the cultured isolates. At the time of illustration, the eukaryotic picoplankton were displayed in large numbers mainly in summer and just only one green algal specie called Micromonas pusilla (prasinophyceae) were more prominent in the community all through the year, and this was done to identify the prominence of picoueukaryotic species in the western English channel.

Science & research, Risk factors, Eukaryotes, Microbiology, Green algae

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