GC fractionation enhances microbial community diversity assessment and detection of minority populations of bacteria by denaturing gradient gel electrophoresis

Article Abstract:

A new strategy that merges the different community analysis approaches by combining GC fractionation and denaturing gradient gel electrophoresis (DGGE) methods is used to enhance the assessment of the microbial community diversity and detect the minority populations of the microbes. The combination of GE fractionation and DGGE method overcomes the individual limitations of each method and permits visualization and recovery of sequences from the minority populations of bacteria.

Usage, Gel electrophoresis, Microbiological techniques (Diagnosis)

---

Effective recovery of bacterial DnA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens

Article Abstract:

A study was conducted to analyze a DNA-based, direct technique for the initial preparation of the total bacterial community in ileum and cecum of the gastrointestinal tract of chickens. Total bacterial DNA samples were determined based on the percent G+C content. The digesta samples were kep on ice and processed within two hours. Experimental results indicated the effectiveness of the technique in examining the culturability of the bacteria.

Research, DNA, Bacteria, Chickens, Gastrointestinal system, Ileum

---

Percent G + C profiling accurately reveals diet-related differences in the gastrointestinal microbial community of broiler chickens

Article Abstract:

The composition of gastrointestinal microflora in chickens can be controlled by diet alone, according to G + C profiling of microbial populations in eight chicken farms in Southern Finland. This means that farmers may be able to eliminate the use of antibiotics for this purpose. The widespread use of antibiotics in food animals is linked to the development of antibiotic resistance in bacteria.

Health aspects, Intestines, Intestinal microbiology, Microbial populations

Similar abstracts:

• Abstracts: Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: comparison by microscopy and denaturing gradient gel electrophoresis
• Abstracts: Antagonistic interactions among marine pelagic bacteria. Actively growing bacteria in the inland sea of Japan, identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis
• Abstracts: Quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus
• Abstracts: Statistical quantification of detachment rates and size distributions of cell clumps from wild-type (PAO1) and cell signaling mutant (JP1) Pseudomonas aeruginosa biofilms
• Abstracts: Generalized transduction of small Yersinia enterocolitica plasmids. Characterization of enterocoliticin, a phage tail-like bacteriocin, and its effect on pathogenic Yersinia enterocolitica strains

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.