Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2

Article Abstract:

A comparison is presented between CelY and CelZ in the production of ethanol when added to fermentation broth for the solubilization of cellulose. Research indicates that fungal enzymes that possess substrate profiles similar to CelY, preferring long-chain polymers and displaying a lack of activity on soluble cello-oligosaccharides between two to five glucosyl residues, may be limiting if used in commercial cellulase preparations.

Statistical Data Included, Bacteria, Pathogenic, Pathogenic bacteria, Klebsiella

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Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli

Article Abstract:

Research describes cloning and gene expression of Klebsiella oxytoca xylodextrin utilization operon, encoding a xylobiose/cation symport, and a new xylosidase in Escherichia coli. The functional expression of the genes in E. coli KO11 demonstrates the metabolism of soluble beta-1,4-linked xylodextrins, which display up to six xylosyl residues.

Microbial metabolism, Biodegradation, Carbohydrate metabolism

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Enhancement of expression and apparent secretion of Erwinia chrysanthemi Endoglucanase (encoded by celZ) in Escherichia coli B

Article Abstract:

Escherichia coli B has been engineered as a biocatalyst for the conversion of lignocellulose into ethanol. Two methods have been developed for improving celZ expression in E. coli B. By screening 18,000 random fragments of Zymomonas mobilis DNA, a surrogate promoter was identified which increased celZ expression up to sixfold. With this promoter, large polar inclusion bodies were clearly evident in the periplasmic space. Addition of the out genes from Erwinia chrysanthemi EC16 caused a further increase in the production of active enzyme.

Escherichia coli, Enzymes, Microbiology, Alcohol, Ethanol, Cellulose

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