Human colonic biota studied by ribosomal DNA sequence analysis

Article Abstract:

Comparison of the ribosomal DNA (rDNA) of bacteria present in human feces with cultured bacteria provides good identification of the human colonic biota. The method involves amplification and sequencing of 16S rRNA genes cloned from the feces. Nine PCR cycles preserve the bacterial diversity and the results are similar to those obtained by direct bacterial culturing. A greater number of PCR cycles distort the diversity. The colonies identified by the method give 21 rDNA sequences which cover 72% of the rDNA of all the culturable cells.

Feces, Microbiology

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Insertion of transposon Tn917 derivatives into the Lactococcus lactis subsp.lactis chromosome

Article Abstract:

The replication of pTV32 and pLTV1 and the transposition of TV32 and LTV1 in Lactococcus lactis subsp. lactis MG1614 are discussed. Temperature shift was initially used on Lactococcus cells transformed with pTV32 or pLTV1. As the method proved to be unsuccessful on the plasmids, transformant growth was used to induce transposition and cure the cells of these plasmids. The utility of transposon LTV1 for studying the molecular biology and physiology of Lactococcus was demonstrated.

Research, Genetic aspects, Plasmids, Gram-positive bacteria

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Establishment of an arbitrary PCR for rapid identification of Tn917 insertion sites in Staphylococcus epidermidis: characterization of biofilm-negative and nonmucoid mutants

Article Abstract:

Research describes an arbitrary polymerase chain reaction (PCR) for rapidly identifying transposon 917 (Tn917) insertion sites in Staphylococcus epidermidis. The methodology uses amplified versions of short chromosomal DNA fragments flanking both ends of the Tn917. Data indicate that the technique identifies Tn917 insertion sites in biofilm-negative and positive and nonmucoid forms of S. epidermidis.

Germany, Methods, Design and construction, Insertion elements, DNA, DNA insertion elements

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