Increase in alfalfa nodulation, nitrogen fixation, and plant growth by specific DNA amplification in Sinorhizobium meliloti

Article Abstract:

Specific DNA amplification (SDA) is an efficient means of enhancing the symbiotic nitrogen fixation capacity of alfalfa plants, as revealed by an experiment conducted on Sinorhizobium meliloti strains. Strains derived from the duplication of the symbiotic region of the plant sample did not only manifest improved symbiotic properties, but exhibited increased nodulation as well. The strains also proved effective in facilitating the growth of alfalfa plants.

Usage, Genetic engineering, Nitrogen, Nitrogen fixation, Alfalfa, Nitrogen-fixing plants

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Efficient procedure for purification of obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple-displacement amplification

Article Abstract:

An efficient and reliable procedure to representatively amplify the Wolbachia genome by multiple-displacement amplification from limited infected host tissue is described. Results indicate that the genome of strain wRi, an obligatory endosymbiont of Drosophila simulans, shares a similar overall architecture with its relative strain wMel.

Analysis, Endosymbiosis, Purification

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Survival of Wolbachia pipientis in cell-free medium

Article Abstract:

The ability of purified Wolbachia bacteria to survive in cell-free media is assessed using BacLight live-dead staining, PCR, and fluorescence in situ hybridization. Results reveal that purified Wolbachia bacteria are able to survive extracellularly for up to 1 week with no decrease in viability.

Science & research, Bacterial growth, Polymerase chain reaction, Report

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