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Biological sciences

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Lysogeny and bacteriocinogeny in Xenorhabdus nematophilus and other Xenorhabdus spp

Article Abstract:

An investigation was conducted to determine the presence of lysogenic phages in Xenorhabdus spp. The results showed that mitomycin or high temperature treatment on X. nematophilus A24 could induce cell lysis with the concomitant production of phages and bacterioicin. Similar results were obtained for other Xenorhabdus spp., except for X. luminescens K80. Furthermore, DNA hybridization experiments demonstrated the presence of prophage genes in the chromosome of A24. These results show that different species of Xenorhabdus harbor lysogenic phages.

Genetic aspects, Enterobacteriaceae, Microorganisms, Lysogeny, Temperature effects

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Fast and accurate identification of Xenorhabdus and Photorhabdus species by restriction analysis of PCR-amplified 16S rRNA genes

Article Abstract:

A restriction analysis of the PCR-amplified 16S rDNA reveals two divergent groups bacterial symbionts of entomopathogenic nematodes. Group I comprises nine genotypes, including all Xenorhabdus species and strains, and symbionts of Steinernema. Group II consists of eight genotypes, including all Photorhabdus strains and symbionts of Heterorhabditis. Amplified 16S rDNA restriction analysis is an efficient and simple method.

Analysis, Usage, Observations, Bacteria, Polymerase chain reaction, Recombinant DNA, Nematoda, Nematodes

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Txp40, a ubiquitous insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria

Article Abstract:

A toxin gene Txp40 was identified in Xenorhabdus and Photorhabdus, which is lethal to the larvae of insects such as Helicoverpa armigera. Histological data from H. armigera larvae into which the toxin was injected suggested that the primary site of action of the toxin is the midgut although some damage to the fat body was also observed.

Science & research, Insects, Bacterial proteins, Microbial toxins

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