Measurements of the distribution of adenylate concentrations and adenylate energy charge across Pseudomonas aeroginosa biofilms

Article Abstract:

A method was developed to measure the adenine nucleotides and adenylate energy charge in a biofilm of Pseudomonas aeroginosa. This entails freezing and sectioning biofilm in a constant-depth film fermentor. Extraction and assaying of the adenylates form the second phase. Results showed that all adenylates increased in concentration toward the surface through time. The dominant adenylate was adenosine monophosphate with relatively higher concentrations at the bottom. Increases of 0.2 units in the energy charge were recorded from the bottom to the surface of the biofilm.

Measurement, Microbial metabolism, Microbial mats, Biomass energy

---

Cloning and characterization of polyphosphate kinase and exopolyphosphatase genes from Pseudomonas aeruginosa 8830

Article Abstract:

The genes encoding polyphosphate kinase (PPK) and exopolyphosphatase (PPX) were isolated from Pseudomonas aeruginosa 8830 to study polyphosphate metabolism and clarify the function of polyphosphate in the microorganism. In contrast to Escherichia coli, the ppk and ppx genes in P aeruginosa are transcribed divergently and no coregulation between their expression was shown by a study of the two promoters. A residual low PPK activity was detected in the mutant, representing a separate mechanism of polyP synthesis.

Genetic aspects, Nucleotide sequence, Base sequence, Promoters (Genetics), Pseudomonas aeruginosa, Biopolymers

---

Similar abstracts:

• Abstracts: Relationship between glycocalyx and povidone-iodine resistance in Pseudomonas aeruginosa (ATCC 27853) biofilms
• Abstracts: Tests of the function of the song repertoire in Bobolinks. Song versatility and social context in the Bobolink
• Abstracts: Cloning of a creatinase gene from Pseudomonas putida in Escherichia coli by using an indicator plate. Chromosomal locus for cadmium resistance in Pseudomonas putida consisting of a cadmium-transporting ATPase and a MerR family response regulator
• Abstracts: Application of DNA probes to analysis of bacteriophage distribution patternsin the environment. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates
• Abstracts: Degradation of 4-chlorophenol via the meta cleavage pathway by Comamonas testosteronii JH5. Pathway of propionate oxidation by a syntrophic culture of Smithella propionica and Methanospirillum hungatei

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.