Molecular epidemiology of Campylobacter jejuni in broiler flocks using randomly amplified polymorphic DNA-PCR and 23S rRNA-PCR and role of litter in its transmission

Article Abstract:

The role of used litters as reservoirs for Campylobacter jejuni in poultry houses has been investigated. The transmission of C. jejuni to successive broiler flocks in the same poultry houses through used litters have also been examined. Genetic typing of recovered C. jejuni isolates was used in tracing strains of the bacteria through successive flocks. Results reveal used litters are not sources of C. jejuni in poultry houses. C. jejuni has been found to be present in the water, litter and dust of poultry houses.

Environmental aspects, Diseases, Campylobacter infections, Campylobacteriosis, Broilers (Poultry), Broilers (Chickens), Poultry houses and equipment, Poultry equipment

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Virulence determinants invA and spvC in Salmonellae isolated from poultry products, wastewater, and human sources

Article Abstract:

The virulence factors in the pathogenesis of Salmonellae isolated from poultry samples, wastewater and human sources were investigated to understand the interaction between salmonellae and the intestinal mucosa. Two virulence foci, invA and spvC, were detected in all three isolates. A significant relationship was observed between invasiveness of the isolates and their source, suggesting that virulence factors have a role in the development of salmonellosis.

Virulence (Microbiology), Salmonellosis

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Molecular genetic relationships of the Salmonellae

Article Abstract:

Ninety-six strains of the salmonellae were subjected to multilocus enzyme electrophoresis. Eighty electrophoretic types were distinguished and placedin eight groups. Data suggested that the taxonomic group VII represents an old, differentiated lineage to which one or more major parts of the chromosmal genome of the group IV lineage have been horizontally transferred. Group V was strongly differentiated from the other groups based on molecular genetic data.

Molecular genetics

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