Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity

Article Abstract:

The Sir2 protein is known to mediate the gene silencing process in budding yeast. This protein, which is also involved in the repression of recombination at the rDNA repeats, is part of a nucleolar complex tagged as RENT, an acronym for regulator of nucleolar silencing and telophase exit. In conjunction with Net1, another subunit of RENT, the Sir2 protein colocalizes to a subdomain within the nucleolus during interphase. However, at the end of mitosis, part of the Sir2 protein moves from the nucleolus to the nucleus, indicating a structural change in the rDNA silent chromatin.

Physiological aspects, Recombinant DNA, Yeast, Yeast (Food product), Mitosis, Nucleolus organizer region

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Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin

Article Abstract:

H2A.Z nucleosomes were found at the promoter regions and generally occurred as two positioned nucleosomes that flanked a nucleosome-free region, which contained the transcription, start site. Enrichment at 5' ends was observed not only at actively transcribed genes but also at inactive loci and mutagenesis of a typical promoter revealed a 22bp segment of DNA sufficient to program formation of a NFR flanked by two H2A.Z nucleosomes.

Science & research, Genetic research, Mutagenesis, Nucleosomes

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Evolution of a combinatorial

Article Abstract:

The plasticity of gene regulation over evolutionary timescales is discussed with respect to mating type transcriptional regulation in yeasts. A compative analysis of the mating behavior between Candida albicans and Saccharomyces cerevisiae reveals that in C. albicans a positive regulator of mating type is retained, whereas it is lost in S. cerevisiae.

United States, Genetic aspects, Comparative analysis, Genetic transcription, Transcription (Genetics), Courtship of animals, Mating behavior, Yeast fungi, Yeasts (Fungi), Chemical evolution, Molecular evolution, Mating behaviour

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