Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair
Article Abstract:
Proteins that are tagged ubiquitin-conjugating enzyme variants (UEV) lack the active-site residues found in the E2 proteins they resemble. A UEV protein that is MMS2-encoded is examined to determine its role in error-free postreplicative DNA repair in Saccharomycetes cerevisiae. It is shown that Mms2p forms a heterodimeric complex with the UBC-13-encoded E2 and is needed for the Ubc 13p-dependent assembly of polyubiquitin chains linked through lysine 63. Results provide support for a model in which an Mms/Ubc 13p complex assembles novel polyubiquitin chains for signaling in DNA repair.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation
Article Abstract:
Hox homeodomain proteins are developmental regulators that determine body plan in several organisms. Most of vertebrate Hox proteins bind DNA as heterodimers with the Pbx1 homeodomain protein. The 2.35 angstrom structure of a ternary complex with a human HoxB1-Pbx1 heterodimer bound to DNA was investigated. Heterodimer contacts are mediated by the hexapeptide of HoxB1, which binds in a pocket in the Pbx1 protein formed in part by a three-amino acid insertion in the Pbx1 homeodomain.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A role for the deubiquinating enzyme USP28 in control of the DNA-damage response. Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints
- Abstracts: A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Mat(alpha)2 repressor degradation
- Abstracts: Inducers of plant systematic acquired resistance regulate NPR1 function through redox changes. The C. elegans zyg-1 gene encodes a regulator of centrosome duplication with distinct maternal and paternal roles in the embryo
- Abstracts: Graded positional information: interpretation for both fate and guidance. Centrosome duplication: a centriolar Pas de Deux
- Abstracts: The pancreatic beta cell heats up: UCP2 and insulin secretion in diabetes. part 2 Endosome-specific localization and function of the ARF activator GNOM