Notch activity influences the alpha-beta versus gamma-delta T cell lineage decision

Article Abstract:

The mechanism involved in the T cell lineage decision was studied using transgenic mice as host systems, supplemented with flow cytometry and polymerase chain reaction-restriction fragment length polymorphism. The results revealed that T cells that are heterozygous for a mutation of the Notch1 gene have a greater tendency to develop as gamma-delta T cells rather than as alpha-beta T cells. A constitutively activated form of Notch gene, on the other hand, stimulates the thymocytes to adopt the alpha-beta T cell fate. Notch, together with the newly formed T cell antigen receptor are thus identified as determinants of T cell lineage decision.

Methods, Usage, Flow cytometry, Polymerase chain reaction, Laboratory animals, T cells, Genetically modified mice, Molecular genetics

---

The EGF-CFC protein one-eyed pinhead is essential for nodal signaling

Article Abstract:

A study was conducted to show that embryos of one-eyed pinhead lacking maternal and zygotic Oep activity are defective in germ layer formation, organizer development, and the positioning of the anterior-posterior axis. The embryos were placed at shield stage and oriented in a lateral view. Several plasmids were then linearized. Moreover, sense stand-capped mRNA were prepared using the mMESSAGE mMACHINE system.

Analysis, Proteins, Ichthyological research, Ichthyology, Embryology, Animal embryology

---

Similar abstracts:

• Abstracts: Ectopic expression of the flagellar regulon alters development of the Bordetella-host interaction. Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid
• Abstracts: Activity of microbial promoters during intracellular and extracellular growth. A 46kDa integral membrane protein from Mycobacterium leprae resembles a number of bacterial and mammalian membrane transport protein
• Abstracts: cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway
• Abstracts: Localization of denitrification genes on the chromosomal map of Pseudomonas aeruginosa. Protein C (OprC) of the outer membrane of Pseudomonas aeruginosa is a copper-regulated channel protein
• Abstracts: Close similarity between Drosophila neurexin IV and mammalian Caspr protein suggests a conserved mechanism for cellular interactions. part 2

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.