P1 trisaccharide (Galalpha1,4Galbeta1,4GlcNAc) synthesis by enzyme glycosylation reactions using recombinant Escherichia coli

Article Abstract:

This paper describes the synthesis of P1 trisaccharide that requires coordination of four enzymes in recombinant Escherichia coli, in which the enzymes are coexpressed. Data indicate that P1 trisaccharide accumulates up to 5.4 gram per 200 mililiter medium with 67% yield.

Canada, Biosynthesis, Recombinant molecules

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Characterization of Streptococcus mutans strains deficient in EIIAB (super)Man of the sugar phosphotransferase system

Article Abstract:

Research has been conducted on EIIAB (super)Man protein of Streptococcus mutans UA159. The role of this protein in carbohydrate transport, in gene expression control and in virulence determinant regulation has been investigated, and the results are reported.

Genetic regulation, Streptococcus mutans, Phosphotransferases

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Enhanced production of alpha-galactosyl epitopes by metabolically engineered Pichia pastoris

Article Abstract:

Research has been conducted on Pichia pastoris. The authors describe the construction of metabolically engineered P. pastoris system for the large-scale production of alpha-galactosyl epitopes with in situ UDP-galactose regeneration.

Japan, Research and Development in the Physical, Engineering, and Life Sciences, Genetic Engineering, Enzymes, Other Basic Organic Chemical Manufacturing, Gene mutations, Gene mutation, Yeast fungi, Yeasts (Fungi), Antigenic determinants

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