P1 trisaccharide (Galalpha1,4Galbeta1,4GlcNAc) synthesis by enzyme glycosylation reactions using recombinant Escherichia coli
Article Abstract:
This paper describes the synthesis of P1 trisaccharide that requires coordination of four enzymes in recombinant Escherichia coli, in which the enzymes are coexpressed. Data indicate that P1 trisaccharide accumulates up to 5.4 gram per 200 mililiter medium with 67% yield.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Characterization of Streptococcus mutans strains deficient in EIIAB (super)Man of the sugar phosphotransferase system
Article Abstract:
Research has been conducted on EIIAB (super)Man protein of Streptococcus mutans UA159. The role of this protein in carbohydrate transport, in gene expression control and in virulence determinant regulation has been investigated, and the results are reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Enhanced production of alpha-galactosyl epitopes by metabolically engineered Pichia pastoris
Article Abstract:
Research has been conducted on Pichia pastoris. The authors describe the construction of metabolically engineered P. pastoris system for the large-scale production of alpha-galactosyl epitopes with in situ UDP-galactose regeneration.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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