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Biological sciences

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Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7

Article Abstract:

Results indicate that the degradation of the 2-nitrobenzoate proceeds via 3-hydroxyanthranilate intermediate mediated by a gene cluster containing nbaC and nbaD in Pseudomonas fluorescens strain KU-7. The nbaC encoded NbaC enzyme is involved in the oxidation of 3-hydroxyanthanilate to 2-amino-3-carboxymuconate-6-semialdehyde, while nbaD encoded NbaD enzyme catalizes decarboxylation of the 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase to 2-aminomuconate-6-semialdehyde.

Canada, Japan, Microbial metabolism, Biodegradation, Microbial enzymes

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Pseudomonad cyclopentadecanone monooxygenase displaying an uncommon spectrum of Baeyer-Villiger oxidants of cyclic ketones

Article Abstract:

A new Baeyer-Villiger monooxygenases (BVMO), cyclopentadecanone monooxygenase (CpdB or CPDMO), originally derived from Pseudomonas sp. strain HI-70 is cloned, sequenced, and over expressed in Escherichia coli. A modest selectivity (E = 5.8), macrolactone formation of 15-hexadecanolide from the kinetic resolution of 2-methylcyclopentadecanone using CPDMO is also demonstrated.

Genetic aspects, Ketones, Structure, Chemical properties, Monoamine oxidase

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Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. strain NCIMB 9871 and localization of the genes that encode them

Article Abstract:

Research is conducted on cyclohexanol degradation pathway gene isolation and characterization to determine the pathway regulation and its biocatalysis potential. Results indicate the location and sequence of a gene that encodes 6-oxohexanoate dehydrogenase activity and identify a transcriptional activator ChnR.

International, Analysis, Metabolism, Gene expression, Dehydrogenases, Oxidoreductases, Microbiological research

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