Purification and structural determination of an inhibitor of Starfish oocyte maturation from a Bacillus species
Article Abstract:
An oocyst maturation inhibiting substance (OMIS) obtained from a bacterial culture has been found to inhibit the 1-methyladenine-induced oocyte maturation but not the dithiothreitol-induced maturation. The OMIS is the lactone of 3-hydroxy-13-methyltetradecanoyl-Glu-Leu-Leu-Val-Asp-Leu-Leu. Though the amino acid configurations have not been determined, the structure and inhibition activity of this OMIS are the same as that of surfactin. OMIS inhibits the oocyte maturation by affecting either the hormone signal transduction or the cell cycle regulation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Purification and properties of ArfI, an alpha-L-arabinofuranosidase from Cytophaga xylanolytica
Article Abstract:
A study was conducted to determine purification and properties of alpha-L-arabinofuranosidase from Cytophaga xylanolytica. Purification was undertaken via anion exchange and hydrophobic interaction column chromatography. Results revealed that ArfI demonstrated zero hydrolytic activity toward a range of p-nitrophenyl- or 4-methylumbelliferyl-glycosides apart from arabinoside. Furthermore, ArfI was generally cytoplasmic which connotes that its activity in vivo is most suitable to hydrolysis of arabinose-containing oligosaccharides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata
Article Abstract:
The beta-glucosidase produced and purified from Candida peltata hydrolyzes cellobiose, shows a synergistic interaction with cellulase and has high glucose tolerance. The enzyme is a monomeric protein with a molecular weight of 43,000, and has maximum activity at 50 degrees celsius and 5.0 pH. The beta-glucosidase has little transglucosylation activity. Divalent cations are unessential for the enzymatic activity which is unaffected by p-chloromercuribenzoate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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- Abstracts: Purification and characterization of a prolidase from Lactobacillus casei subsp. casei IFPL 731. Growth inhibition of metronidazole-susceptible and metronidazole-resistant strains of Gardnerella vaginalis by lactobacili in vitro
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