Quantification of ergosterol and 3-hydroxy fatty acids in settled house dust by gas chromatography-mass spectrometry: comparison with fungal culture and determination of endotoxin by a Limulus amebocyte lysate assay

Article Abstract:

The quantification of ergosterol and 3-hydroxy fatty acids (3-OH FAs) in settled house dust by gas chromatography-mass spectrometry was compared with fungal culture while endotoxin by a Limulus amebocyte lysate assay was studied to develop a method for measuring endotoxin and ergosterol in dust. The research used a sample analysis approach that provided an extraction procedure that allowed the measurement of both ergosterol and 3-OH FAs in a single dust sample. Results reveal a wide distribution of 3-OH FA lengths in house dust.

Endotoxins, Fatty acids

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Application of gas chromatography-mass spectrometry for rapid detection of Mycobacterium xenopi in drinking water

Article Abstract:

Gas chromatography-mass spectrometry (GC-MS) was used to detect 2-docasonol, a chemical marker for Mycobacterium xenopi, and tuberculostearic acid (TSA), a chemical marker for mycobacteria and related species, in drinking water. The results from the GC-MS method agreed well with results obtained by standard culturing techniques. This indicates that GC-MS is a useful technique for the rapid detection of M. xenopi and other mycobacterial species in drinking water.

Health aspects, Identification and classification, Mycobacterium, Drinking water

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Characterization of the microbial community in indoor environments: a chemical-analytical approach

Article Abstract:

Research has been conducted on microbial flora of indoor environments. The authors describe the use of gas chromatography-ion trap mass spectrometry to determine chemical markers of gram-positive bacteria, fungal biomass, bacterial peptidoglycan and gram-negative bacterial lipopolysaccharide in settled house dust samples.

Sweden, Science & research, Analytical Laboratory Instrument Manufacturing, Analytical instruments, Ion Mass Spectroscopy, Physiological aspects, Demographic aspects, Environmental aspects, Genetic aspects, Gram-negative bacteria, Microbiology, Peptidoglycans, Biomass, Microbiological chemistry, Gram-positive bacteria, Microbial populations, Microbial ecology

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