Quantification of ergosterol and 3-hydroxy fatty acids in settled house dust by gas chromatography-mass spectrometry: comparison with fungal culture and determination of endotoxin by a Limulus amebocyte lysate assay
Article Abstract:
The quantification of ergosterol and 3-hydroxy fatty acids (3-OH FAs) in settled house dust by gas chromatography-mass spectrometry was compared with fungal culture while endotoxin by a Limulus amebocyte lysate assay was studied to develop a method for measuring endotoxin and ergosterol in dust. The research used a sample analysis approach that provided an extraction procedure that allowed the measurement of both ergosterol and 3-OH FAs in a single dust sample. Results reveal a wide distribution of 3-OH FA lengths in house dust.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Application of gas chromatography-mass spectrometry for rapid detection of Mycobacterium xenopi in drinking water
Article Abstract:
Gas chromatography-mass spectrometry (GC-MS) was used to detect 2-docasonol, a chemical marker for Mycobacterium xenopi, and tuberculostearic acid (TSA), a chemical marker for mycobacteria and related species, in drinking water. The results from the GC-MS method agreed well with results obtained by standard culturing techniques. This indicates that GC-MS is a useful technique for the rapid detection of M. xenopi and other mycobacterial species in drinking water.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Characterization of the microbial community in indoor environments: a chemical-analytical approach
Article Abstract:
Research has been conducted on microbial flora of indoor environments. The authors describe the use of gas chromatography-ion trap mass spectrometry to determine chemical markers of gram-positive bacteria, fungal biomass, bacterial peptidoglycan and gram-negative bacterial lipopolysaccharide in settled house dust samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Genetic population structure and gene flow in the Atlantic code Gadus morhua: a comparison of allozyme and nuclear RFLP loci
- Abstracts: Distribution of Vibrio vulnificus in the Chesapeake Bay. Iasolation and diversity of actinomycetes in the Chesapeake Bay
- Abstracts: Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway. Degradation of 2-methylbenzoic acid by Pseudomonas cepacia MB2
- Abstracts: Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells
- Abstracts: Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay