Enterococcus faecalis gene transfer under natural conditions in municipal sewage water treatment plants

Article Abstract:

Different strains of Enterococcus faecalis found in two sewage water treatment plants in the Bavarian cities of Munich and Regensburg were examined with particular focus on their ability to transfer a number of genetic elements under natural conditions. The findings indicate that, compared to gene transfer in laboratory conditions, the transfer rate between different strains under natural conditions was at least 10-fold lower for Tn916, at least 100-fold lower for pIP501 and at least 10(super 5)-fold lower for the sex pheromone plasmids.

Author: Muscholl-Silberhorn, Albrecht, Wirth, Reinhard, Marcinek, Herbert, Gauer, Matthias
Sewage disposal plants, Aquatic microbiology, Bacterial transformation, Genetic transformation

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Influence of various dust sampling and extraction methods on the measurement of airborne Endotoxin

Article Abstract:

Studies done on airborne samples from a potato processing plant shows that the extraction of bacterial endotoxins is less when the filters used are either of cellulose containing esters or of polypropylene. This is because these chemicals bind the endotoxins. With every freeze-thaw cycle, the amount of endotoxins extracted was less, showing that freezing and thawing affects the extraction rather than the duration of storage. However, temperature and rocking of the sample do not appear to affect the recovery amount.

Author: Douwes, Jeroen, Doekes, Gert, Versloot, Pieter, Hollander, Albert, Heederik, Dick
Methods, Endotoxins, Extraction (Chemistry)

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Subjects list: Research
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