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Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance

Article Abstract:

The authors discuss their study of Thermus sp. strain IM6501. They report the generation of thermostable maltogenic amylases from this strain via random mutagenesis, and the analysis of the factors associated with the enzyme's thermostability.

Author: Young-Wan Kim, Ji-Hye Choi, Jung-Wan Kim, Cheonseok Park, Jung-Woo Kim, Hyunju Cha, Soo-Bok Lee, Byoung-Ha Oh, Tae-Wha Moon, Kwan-Hwa Park
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
South Korea, Bacteria, Amylases, Mutagenesis

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Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype Enteritidis in poultry

Article Abstract:

Research has been conducted on Salmonella enterica serotype Enteritidis. The authors describe the method for detecting this bacterium in poultry samples, and report that SYBR green I real-time PCR is an effective detection method.

Author: De Medici, Dario, Croci, Luciana, Delibato, Elisabetta, Di Pasquale, Simona, Filetici, Emma, Toti, Laurs
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Italy, Analysis, Usage, Polymerase chain reaction, Salmonella, Methodology, Research methods

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Subjects list: Research, Physiological aspects, Environmental aspects, Genetic aspects, DNA, Microbiology, Microbial populations, Microbial ecology
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