Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni

Article Abstract:

An in vitro transposon-based signature-tagged mutagenesis (STM) system is developed for Campylobacter jejuni and used to identify genes involved in colonization of the gastrointestinal tract of 2-week-old chickens. The findings indicate that the nature of campylobacter colonization in chickens is complex and dynamic, and these observations are presumably a consequence of bottlenecks in the colony establishment process.

Transposons, Mutagenesis

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Phase-Variable surface structures are required for infection of Campylobacter jejuni by bacteriophages

Article Abstract:

The interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme is characterized by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. It is shown that the 16 typing phages fall into four groups based on their patterns of activity against spontaneous mutants.

Bacteriophages

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Protein engineering of Wzc to generate new emulsan analogs

Article Abstract:

Protein engineering methods were used to generate the emulsan analogs. The apoemulsan polysaccharide-copolymerase (PCP) protein (Wzc) affects the molecular weight of a high-molecular weight polysaccharide.

Protein engineering, Microbial genetics, Report

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