Transposon mutagenesis of the obligate intracellular pathogen rickettsia prowazekii

Article Abstract:

The problems by adapting a gene that codes for rifampin resistance for expression in R. prowazekii and by incorporating this selection into a transposon mutagenesis system suitable for generating rickettsial gene knockouts are addressed. Transposomes provide a mechanism for generating random insertional mutations in R. powazekii, thereby identifying nonessential rickettsial genes.

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Multiplex PCR assay for detection of bacterial pathogens associated with warm-water streptococcosis in fish

Article Abstract:

A multiplex PCR-based method is developed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish. The conclusion states that multiplex PCR assay is useful for the specific detection of the four species of bacteria in pure culture as well as in inoculated fish tissue homogenates and naturally infected fish.

Streptococcal infections, Risk factors

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mariner-based transposon mutagenesis of Rickettsia prowazekii

Article Abstract:

The versatile mariner-based Himar1 transposon system is used to generate insertional mutants of Rickettsia prowazekii.

Genetic aspects

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