Spiralin is not essential for helicity, motility, or pathogenicity but is required for efficient transmission of Spiroplasma citri by its leafhopper vector Circulifer haematoceps
Article Abstract:
Results show that mutants of Spiroplasma citri ,devoid of the surface protein spiralin, exhibit helical morphology and motility as those of wild-type S. citri. Further, the mutants when injected into the insect vector multiply but get transmitted to the host plant 100 times less efficiently. Taken together, data suggest that spiralin is not required for pathogenicity but is required for efficient transmission of S. citri by the insect vector.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Disruption of a gene predicted to encode a solute binding protein of an ABC transporter reduces transmission of spiroplasma citri by the leafhopper Circulifer haematoceps
Article Abstract:
The spiroplasma citri is spread from one plant to the other with the help of the leafhoppers, which survive on the phloem. The mutants, which are affected by the leafhopper in this transmission process, are called Circulifer haematoceps and this is chosen after the completion of the transmission to periwinkle plants.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Fructose operon mutants of Spiroplasma citri
Article Abstract:
Two methods for selecting fructose-negative mutants of Spiroplasma citri are described. One is based on selecting xylitol-resistant mutants, and the other is based on inactivating fruA or fruK by homologous recombination.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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