Stage-specific regulation of murine Hsp68 gene promoter in preimplantation mouse embryos

Article Abstract:

Endogenous hsp68 expression regulates development of hsp68-lacZ, a fusion gene which acts as an inducible heat shock gene in transfected mouse L-cells, after genome integration in transgenic preimplantation embryos. Normally cleaving embryos are used to explore the developmental activity of promoter sequences obtained from hsp68, which is spontaneously and transiently expressed at the start of embryonic transcription. Fusion gene activity may be controlled by proximal sequences at the two-cell stage and by enhancer sequences at the four-cell stage. Heat shock elements are not needed at early stages, but are required at late stages before implantation.

Genetic regulation, Heat shock proteins, Promoters (Genetics), Developmental genetics

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Developmental and tissue-specific regulation of the murine cardiac actin gene in vivo depends on distinct skeletal and cardiac muscle-specific enhancer elements in addition to the proximal promoter

Article Abstract:

Transgenic experiments were carried out to determine the role of the cardiac actin gene in muscle development in the mouse. High-level expression of the cardiac actin gene in the skeletal muscle of transgenic mice involved distal and proximal enhancer sequences and the proximal promoter sequences. It also generated an nlacZ reporter gene. It is suggested that expression of the cardiac actin gene requires a cardiac enhancer located in between the proximal enhancer and proximal promoter regions.

Actin, Heart muscle, Myocardium

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Redefining the genetic hierarchies controlling skeletal myogenesis: Pax-3 and Myf-5 act upstream of MyoD

Article Abstract:

Sp homozygous mice were used as experimental models to study the programming of skeletal myogenesis. The results of the loss-of-function approach demonstrated that the bHLH myogenic regulatory factor Myf-5 and the paired box transcription factor Pax-3 are required by progenitor cells to generate muscles. Another bHLH myogenic regulatory factor, MyoD, on the other hand, was found to act genetically downstream of both Pax-3 and Myf-5 for the establishment of skeletal muscles in the body.

Analysis, Usage, Genetic transcription, Transcription (Genetics), Laboratory animals, Immunofluorescence, Fluorescent antibody technique, Myogenesis

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