Strain typing with IS200 fingerprints in Salmonella abortusovis

Article Abstract:

Hybridization profiles of the IS200 insertion element of Salmonella abortusovis strains isolated from sheep reveal a band at almost 9 kb, besides additional strain-specific bands. The isolates from Sardinia, Italy, show low polymorphism and three IS200 hybridization bands. Most of the strains contain a copy of IS200 in the variable area of their virulence plasmid. Strain typing using IS200 fingerprinting is more accurate than chromosome restriction fragment length polymorphism typing, plasmid profiles and rRNA restriction fragment length polymorphism analysis.

Usage, DNA testing, DNA identification, Insertion elements, DNA, DNA insertion elements

---

Identification of Salmonella abortusovis by PCR amplification of a serovar-specific IS200 element

Article Abstract:

A polymerase chain reaction assay was developed for the identification of Salmonella abortusovis DNA based on the presence of the serovar-specific IS200 copy on the bacterial chromosome. The serovar-specific IS200 element in Salmonella abortusovis was detected in the 900-bp and 2-kb fragment by Southern hybridization against plasmids pIZ46 and pIZ47. Furthermore, analysis of the fragments from primer combinations indicated the presence of an extra DNA that is located on one side of the IS200 element.

Research, Identification and classification, Polymerase chain reaction, Bacterial genetics, Bacteriology

---

Cost-effective application of pulsed-field gel electrophoresis to typing of Salmonella enterica serovar Typhimurium

Article Abstract:

The relationship between phage type and pulsed-field gel electrophoresis (PFGE) pattern in Salmonella enterica serovar Typhimurium isolates from humans, animals and food in Ireland is explored to determine how to apply PFGE to supplement the existing routine phenotypic subtyping of serovar Typhimurium. The analysis facilitates the selective application of PFGE to maximize the yield of epidemiologically relevant information while controlling costs.

Methods, Gel electrophoresis, Bacteriophage typing

Similar abstracts:

• Abstracts: Mapping the mutation causing lens luxation in several terrier breeds
• Abstracts: Mate protection and winter pair-bonds in Black-capped Chickadees. Impact of tent caterpillar defoliation on the reproductive success of black-capped chickadees
• Abstracts: BRI-ghtening the pathway to steroid hormone signaling events in plants. HOOKLESS1, an ethylene response gene, is required for differential cell elongation in the Arabidopsis hypocotyl
• Abstracts: Breeding biology of the Dolphin Gull at Punta Tombo, Argentina. Satellite tracking of Magellanic Penguin migration
• Abstracts: The regulation of antibiotic production in Streptomyces coelicolor A3(2). Medium- and long-chain fatty acid uptake and utilization by Streptomyces coelicolor A3(2): first characterization of a Gram-positive bacterial system

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.