Transposition of IS117 of Streptomyces coelicolor A3(2) in Mycobacterium smegmatis

Article Abstract:

It was shown that the Streptomyces coelicolor A3(2) 2.6 kb minicircle, IS117, transposes into at least three chromosal attachment sites in Mycobacterium smegmatis mc(super 2)155. The Mycobacterium smegmatis strain was prepared in 7H9 broth. Genomic DNA was then extracted from the strain using the salting-out technique. Moreover, plasmid DNA four double-strand sequencing was purified using the Qiagen plasmid purification kit.

Mycobacterium

---

Medium- and long-chain fatty acid uptake and utilization by Streptomyces coelicolor A3(2): first characterization of a Gram-positive bacterial system

Article Abstract:

The fatty acid uptake of a Gram-positive bacterium, Streptomyces coelicolor A3(2) was characterized. The bacteria consumes fatty acids of C4-C18 chain lenghts as sole carbon and energy sources. Uptake of medium-chain fatty acid octanoate exhibited characteristics of simple diffusion while, uptake of the long-chain fatty acid palmitate, occurred by simple diffusion and active transport.

Fatty acids

---

Similar abstracts:

• Abstracts: Growth rate control of protein and nucleic acid content in Streptomyces coelicolor A3(2) and Escherichia coli B/r
• Abstracts: Demonstration of high-affinity Mn2+ uptake in Saccharomyces cerevisiae: specificity and kinetics. Mixed sulphate-reducing bacterial cultures for bioprecipitation of toxic metals: factorial and response-surface analysis of the effects of dilution rate, sulphate and substrate concentration
• Abstracts: The influence of weather, geography, and habitat on migrating raptors on Cape May peninsula. Effects of oiling on feeding behavior of Sanderlings and Semipalted Plovers in New Jersey
• Abstracts: A fast method for the confirmation of fecal streptococci from M-Enterococcus medium. Presumptive fecal streptococci in environmental samples characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis
• Abstracts: Short-range signaling by candidate morphogens of the TGFbeta family and evidence for a relay mechanism of induction

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.