Streptomyces akiyoshiensis differs from other Gram-positive bacteria in the organization of a core biosynthetic pathway gene for aspartate family amino acids

Article Abstract:

A partial Sau3AI digest of genomic DNA from Streptomyces akiyoshiensis was cloned in a Streptomyces-Escherichia coli shuttle vector, and the recombinant plasmids were used to transform E. coli CGSC 6212, which has a mutation for aspartate semialdehyde dehydrogenase, to study the function of the core reactions in the biosynthesis of aspartate family amino acids and related secondary metabolites. Findings indicated that the asd and ask genes in S. akiyoshiensis are not found in an operon.

Dehydrogenases, Oxidoreductases

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Regulation of an anthranilate synthase gene in Streptomyces venezuelae by a trp attenuator

Article Abstract:

A study was conducted to examine the role of a trp attenuator in the regulation of an anthranilate synthase gene in Streptomyces venezuelae. Results involving cultures of a trpC mutant of the S. venezuelae showed a decline in the formation of anthranilate synthase following an increase in the formation of exogenous tryptophan. The bacteria's ORF2 resembled genes for phenazine biosynthesis in Pseudomonas.

Bacterial genetics, Microbial enzymes

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Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces venezuelae: evidence that it is not required for chlorination in chloramphenicol biosynthesis

Article Abstract:

The genomic DNA libraries of S. venezuelae and a mutant strain are hybridized with a synthetic oligonucleotide. The bromoperoxidase-catalase gene in Streptomyces venezuelae is sequenced and cloned. Analysis of the nucleotide sequences shows no differences in the sequences of the gene and the synthetic oligonucleotide. This indicates that the gene's catalase activity governs its role in S. venezuelae.

Nucleotide sequence, Base sequence, Clone cells

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