Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification
Article Abstract:
A study was conducted to demonstrate that surface plasmon resonance imaging (SPRI) could be used to achieve rapid and high-throughput monitoring of affinity-tagged proteins in the expression and purification processes. It was concluded that the SPRI protein chip system could be used for fast monitoring of a six-His-tagged protein in the expression and purification processes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Expression of heteropolymeric ferritin improves iron storage in Saccharomyces cerevisiae
Article Abstract:
Research demonstrates enhancement of iron-storing capacity of the recombinant yeast through the expression of heteropolymeric apoferritin. The method employs differentially expressing the H and L subunits of human ferritin, which coassemble into a heteropolymer in vivo leading to enhanced iron-carrying capability in yeast.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids
Article Abstract:
The construction of two recombinant reaction systems for the production of optically pure D-amino acids from different D,L-5-monosubstituted hydantoins is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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