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Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae

Article Abstract:

An investigation was conducted to identify genes functionally related to SNF1 which are involved in glucose repression in Saccharomyces cerevisiae. SNF1 codes for a protein-serine/threonine kinase required for the release of gene expression from glucose repression. Two genes, MSI1 and PDE2, from the cAMP-dependent protein kinase pathway (cAPK) were identified in addition to seven other genes. Multicopy dosage of the cAPK genes did not restore invertase expression. Furthermore, constitutive cAPK mutants were still dependent on glucose regulation and SNF1 functions, indicating that cAPK is not required for invertase expression.

Author: Yang, Xiaolu, Carlson, Marian, Hubbard, E. Jane Albert
Publisher: Genetics Society of America
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
Protein kinases, Glucose metabolism

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Identification and characterization of three genes that affect expression of ADH2 in Saccharomyces cerevisiae

Article Abstract:

Three new genes, coding for ADR7, ADR8 and ADR9, from Saccharomyces cerevisiae were identified and characterized initially using a novel selection protocol. The three loci influences the transcription of alcohol dehydrogenase isozyme II (ADH2) gene. The phenotypes of the mutant strains were similar although one strain was shown to be temperature sensitive. The mutants permitted incomplete glucose repression escape of the gene for ADH2. Other characteristics of the mutation, especially those concerning enzymes sensitive to glucose repression, are discussed.

Author: Karnitz, Larry, Morrison, Michael, Young, Elton T.
Publisher: Genetics Society of America
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992

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A mutant tRNA affects delta-mediated transcription in Saccharomyces cerevisiae

Article Abstract:

New extragenic suppressors of mutations in the SPT3, SPT7, SPT8 and SPT15 gene called rsp, for reverses Spt negative phenotype, were isolated and analyzed. The new mutations alters the expression of delta insertions in spt positive strains. RSP genes so far identified numbers four. They encode frameshift mutations in glycine transfer RNA suppressor. Other genetic properties of the new mutations are discussed.

Author: Winston, Fred, Happel, Anne M.
Publisher: Genetics Society of America
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
Transfer RNA

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Subjects list: Research, Genetic aspects, Genetic regulation, Saccharomyces
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