Glyceraldehyde-3-phosphate dehydrogenase is negatively regulated by ADP-ribosylation in the fungus Phycomyces blakesleeanus

Article Abstract:

Results demonstrate that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the substrates of the arginine-specific ADP-ribosyltransferase in the dormant spores of Phycomyces blakesleeanus. Furthermore, ADP-ribosylation inhibits GAPDH but glyceraldehyde 3-phosphate can prevent this inhibition.

Mexico, Enzymes, Dehydrogenases, Oxidoreductases, Enzyme regulation

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The GTP-binding protein G-alpha(sub s) is present in dormant spores and expressed differentially during spore germination of the fungus Phycomyces blakesleeanus

Article Abstract:

The dormant spores of the fungus Phycomyces blakesleeanus contain the stimulatory alpha-subunit of GTP-binding protein, G-alpha(sub s), which is expressed during germination and development. The protein exists until the hyphae formed by the spores branches. The 56 kDa GTP-binding protein was identified in the spores by photoaffinity tests, ADP-ribosylation with cholera toxin and an antibody against mammalian G-alpha(sub s). The fungal G-alpha(sub s) contains amino acid sequences similar to those in rat G-alpha(sub s) gene.

Analysis, Growth, G proteins

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Evidence for an arginine-specific mono(ADP-ribosyl)transferase in dormant spores of the fungus Phycomyces blakesleeanus

Article Abstract:

The role of mono-ADP-ribosylation in Phycomyces blakesleeanus was investigated using dormant spores of the fungus. Two-dimensional electrophoresis identified three different substrates with molecular masses of 36, 37 and 38 kDa and isoelectric potential of 4.6, 8.1 and 6.9, respectively. ADP-ribosylation of these substrates with [32P]NAD and subsequent incubation with hydroxylamine caused the release of radioactivity. Addition of 10 millimolars of MgCl2 stimulated enzymatic activity and increased radioactivity in the presence of GTP or ATP.

Research, Germination, Spores, Dormancy (Biology)

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