The acquisition of indigenous plasmids by a genetically marked pseudomonad population colonizing the sugar beet phytosphere is related to local environmental conditions
Article Abstract:
The transfer of naturally occurring conjugative plasmids from the indigenous microflora to a genetically modified population of bacteria colonizing the phytospheres of plants was investigated. Findings showed that conjugative gene transfer between bacterial populations in the phytosphere may be a common event under specific environmental conditions. The plasmids obtained in situ by the colonizing inocula were identified as natural variants of restriction digest pattern group I, III, or IV plasmids from five genetically distinct groups of large, conjugative mercury resistance plasmids known to persist in the phytospheres of sugar beets at field site.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Impact of plasmid pQBR103 acquisition and carriage on the phytosphere fitness of Pseudomonas fluorescens SBW25: burden and benefit
Article Abstract:
The phytosphere population densities of Pseudomonas fluorescens SBW25EeZY6KX (lacZX aph xylE) carrying pQBR103 decreased substantially compared to plasmid-free populations after seed inoculation. As the sugar beet plants matured, ca. 100 days after planting, simultaneous selections for plasmid-carrying hosts were found in the phyllospheres and rhizospheres of field-grown plants. The recovery of these populations to densities indistinguishable from the densities of plasmid-free inocula shows that phytosphere-associated plasmids confer a specific fitness advantage to host bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Plasmid stability in Pseudomonas fluorescens in the rhizosphere
Article Abstract:
A study was conducted to examine plasmid stability in the root-colonizing strain Pseudomonas fluorescens WCS365. Stability was tested in several laboratory media and in the rhizospheres of various plant species. The plasmids pVSP41 and pWTT2081, which carry the rep and sta regions of the native Pseudomonas aeruginosa, were found to be important as stable vectors for the functional analysis of genes affecting root colonization, as long as control cells hold the empty vector.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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