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Biological sciences

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The gut of the soil microarthropod Folsomia candida (Collembola) is a frequently changeable but selective habitat and a vector for microorganisms

Article Abstract:

Folsomia candida (collembola) is a soil microarthropod that has a dense layer of bacterial cells in its gut. It is an ideal model for investigating the interaction between microorganisms and terrestrial ecosystems. The effect of feeding patterns on the microbial population in the collembolan gut was determined using bacterial cells that were tagged with marker genes. The tags helped trace the passage of the ingested cells through the collembola's alimentary system. Results suggest these organisms were able to colonize the environment. They also eliminated other species efficiently.

Host-bacteria relationships, Soil biology, Collembola, Springtails

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Nisin resistance distinguishes Mycoplasma spp. from Acholeplasma spp. and provides a basis for selective growth media

Article Abstract:

Nisin, a cationic polypeptide, suppresses the growth of Acholeplasma spp. whereas Mycoplasma spp. are resistant to it. Nisin activates glucose oxidation and suppresses respiration in Acholeplasma laidlawaii and A. oculi. The suppression of oxygen intake is more in A. laidlawaii cells grown in the absence of cholesterol. The growth suppression by nisin decreases with increasing concentrations of cholesterol in cells grown on agar. Nisin resistance is important for the selection and identification of Mycoplasma spp.

Growth, Polypeptides, Mycoplasma

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A PCR technique based on the Hip1 interspersed repetitive sequence distinguishes cyanobacterial species and strains

Article Abstract:

Primers based on the Hip1 sequence have been used as a typing technique for cyanobacteria. PCR-based methods for typing have been developed over the years. An octameric palindromic sequence Hip1 is present in the chromosomal DNA of many cyanobacteria. PCR primers were used to extend the Hip1 sequence and five produced different products when used to prime PCR from cyanobacterial genomic DNA. RFLP and Hip1 typing produced similar results and each technique could distinguish different strains.

Cyanobacteria

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