The mercury resistance operon of the IncJ plasmid pMERPH exhibits structural and regulatory divergence from other Gram-negative mer operons

Article Abstract:

The DNA sequence of the pMERPH mer determinant is determined to make possible a comparison with other mer determinants that have currently been sequenced. The scope of the research effort includes the regulation of the pMERPH mer operon and the identification of other pMERPH-like sequences in the natural environment using PCR. The genes merT, merP, merC and merA were identified as a result of the comparison. The Tn21-like determinant of SE20's MerR protein is found to both partially repress expression from the mer P sub TCPA promoter and induce expression in the presence of inducing levels of Hg super 2+.

Bacteria, Operons

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Distribution of class II transposase and resolvase genes in soil bacteria and their association with mer genes

Article Abstract:

There is widespread distribution of Tn501- and Tn21-associated transposase and resolvase gene sequences in both pristine and mercury polluted soil surroundings. There is extensive recombination both between different transposon genes and between transposon and mer genes within the natural populations of soil bacteria. As seen from a study on transposase and resolvase regions, hybrid transposons and the uncharacterized Hg(super r)-associated transposable elements are also present within these.

Genetic aspects, Gram-negative bacteria, Transposons, Ir genes

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Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction

Article Abstract:

A method to amplify DNA from native populations of soil bacteria was developed. The procedure employed polymerase chain reaction techniques using either 'universal' eubacterial 16S rRNA gene primers or primers for mercury resistance genes. Actual tests showed that the procedure could amplify DNA sequences from as little as five micrograms of soil. Confirmation of amplified gene sequences was carried out by Southern hybridization analysis.

Methods, Usage, Soil microbiology, Polymerase chain reaction, Gene amplification

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