The unique 16S rRNA genes of piezophiles reflect both phylogeny and adaptation
Article Abstract:
The isolation and characterization of the first hyperpiezophiles obtained from the southern hemisphere and from another trench environment of the first isolation of piezophilic gram-positive bacteria is presented. The results suggest that the circumpolar deep water acts as repository for hyperpiezophiles and drives their dissemination to deep trenches in the Pacific Ocean and the occurrence of elongated helices in the 16S rRNA genes increases with the extent of adaptation to growth at elevated pressure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
Article Abstract:
The utility of the Greengenes data assembly process and examination of the validity of prokaryotic candidate phyla was illustrated by aligning and chimera checking more than 90,000 public 16S small-subunit rRNA gene sequences. Putative chimeras were identified in 3 percent of environmental sequences, which were classified into 100 phylum-level lineages in Archea and Bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Effects of target length on the hybridization efficiency and specificity of rRNA-based oligonucleotide microarrays
Article Abstract:
The effects of differential rRNA length on the efficiencies of hybridization and specificity of oligonucleotide microarrays were analyzed using fluorescence techniques. The fragmented rRNAs were shown to have improved hybridization efficiency.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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