Tracking RNA polymerase, one step at a time
Article Abstract:
RNA polymerases (RNAPs) transcribe genetic material by selecting neculeoside triphosphate substrates according to the sequence of the DNA template. It is found that individual molecules of bacterial RNA polymerase move in single base-pair steps and concludes that RNA polymerase acts as a Brownian ratchet that is driven forward by the binding of incoming nucleoside triphosphates.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Structural basis for substrate selection by T7 RNA polymerase.
Article Abstract:
The structure of T7 RNA polymerase (RNAP) elongation complex with the incoming substrate analog is determined to study the substrate selection mechanism in RNA polymerases. The structure suggests a novel mechanism, in which the substrates selection occurs prior to the isomerization to the catalytically active conformation.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins
Article Abstract:
The 2.5 angstrom resolution structures of rifabutin and rifapentin complexed with the Thermus thermophilus RNA polymerase (RNAP) holoenzyme are examined. The transmission of a rifamycin-induced signal over 19 angstrom to the RNAP active site is useful in slowing down catalysis.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: RNA polymerase unveiled. Phosphorylation of histone H3 at Ser10 facilitates RNA polymerase II release from promoter-proximal pausing in Drosophila
- Abstracts: Aging in vertebrates, and the effect of caloric restriction: a mitochondrial free radical production-DNA damage mechanism?
- Abstracts: Diurnal variation of cell proliferation in three bacterial taxa from coastal North Sea waters. Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and Archaea in the deep ocean
- Abstracts: Pharmacologic disruption of polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells
- Abstracts: Genome-wide mRNA surveillance is coupled to mRNA export. The role of protein arginine methylation in the formation of silent chromatin