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Biological sciences

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Transcription of the glnB and glnA genes in the photosynthetic bacterium Rhodospirillum rubrum

Article Abstract:

The glnB and glnA genes of the photosynthetic bacteria Rhodospirillum rubrum are co-transcribed from a NtrC-activated sigma(super 54) -dependent promoter. The promoter is present at -13/ -25 bp from the transcription initiation site and contains NtrC binding regions. The genes are also transcribed by a NtrC-independent promoter and processing of the glnBA transcript produces the glnA mRNA. The transcription is regulated by the nitrogen status, and the gene products are more abundant in nitrogen-fixing cells as compared to the cells grown in ammonia.

Genetic regulation, Promoters (Genetics), Genetic transcription, Transcription (Genetics), Bacteria, Photosynthetic, Photosynthetic bacteria

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Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133:glnB or the P(sub II) protein may be essential

Article Abstract:

A study was conducted to test whether Nostoc punctiform P(sub II) proteins influence pathways regulating cellular differentiation and nitrogen metabolism. Conventional techniques were utilized to carry out small-scale plasmid DNA preparations from Escherichia coli while a Bio-Rad Mini-Protean system was utilized to perform native and SDS-PAGE immunoblotting. Results showed a second copy of glnB was needed to disrupt the glnB gene of N. punctiform by targeted gene replacement.

Genetic aspects, Escherichia coli, Proteins, Nitrogen (Chemical element), Nitrogen, Cell physiology

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Nitrogen -starvation-induced chlorosis inSynechococcus PCC 7942: Adaptation to long-term survival

Article Abstract:

Cyanobacteria are widespread Gram-negative bacteria displaying morphological diversity. The non-diazotrophic cyanobacterium Synechococcus sp. strain PCC 7942 undergoes a proteolytic degradation of the phycobiliproteins when deprived of essential nutrients.The degradation is thought to be part of an acclimation process where growing cells differentiate into non-pigmented cells that can endure prolonged starvation.

Observations, Microbiological research, Chlorosis (Plants)

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