Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae
Article Abstract:
Previous studies have reported that gonococci undergo DNA-mediated transformation as a means of chromosomal DNA transfer, and that uptake of DNA is correlated with the production of pilin. To investigate the relationship between DNA transformation efficiency and piliation, transformation was performed using piliated (P+) gonococci and cloned DNA containing a heterologous cat insertion. Addition of excess gonococcal transformation uptake sequence (GCUS) inhibited transformation under conditions of low DNA concentrations but not high DNA concentrations. GCUS also had no effect on the transformation of nonpiliated, pilin-producing P+ strains. These results suggest that transformation may occur in a GCUS-dependent and -independent mechanism.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Arginine-, hypoxanthine-, uracil-requiring isolates of Neisseria gonorrhoeae are a clonal lineage within a non-clonal population
Article Abstract:
The arginine-, hypoxanthine-, uracil-requiring (AHU-) isolates of Neisseria gonorrhoeae satisfy the criteria of a relatively long-lived clone which are found within a basically non-clonal population. The striking uniformity of AHU- isolates differs from the previous findings which included very few AHU- strains and demonstrated the lack of association between auxotype, serovar and genetic background. Diversification of gonococci occurs rapidly due to frequent recombination. Findings revealed that there is no major defect in the ability of AHU- strains to be transformed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Representational difference analysis of cDNA for the detection of differential gene expression in bacteria: development using a model of iron-regulated gene expression in Neisseria meningitidis
Article Abstract:
This paper describes adoption of eukaryotic representational difference analysis of cDNA technique for prokayotic bacterial systems. Identification of five major gene products, involved in the iron-regulation in Neisseria meningitidis, using this technique validates its application to bacterial systems.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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