Use of inducible feedback-resistant N-acetylglutamate synthetase (argA) genes for enhanced arginine biosynthesis by genetically engineered Escherichia coli K-12 strains
Article Abstract:
The argA genes from the three feedback-resistant (FBR) N-acetylglutamate synthetase Escherichia coli K-12 strains were incorporated into previously engineered arginine-producing systems to construct E. coli strains capable of enhanced arginine production without compromising ornithine biosynthesis. An investigation of the modified expression systems in terms of arginine production and strain stability showed that the system with fbr argA produced 7- to 35-fold more arginine and plasmids containing wt or fbr argA were stably maintained under normal growth conditions for at least 18 generations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Enhanced production of arginine and urea by genetically engineered Escherichia coli K-12 strains
Article Abstract:
The underexpression of the chromosomal arginine regulon and the overexpression of the Escherichia coli argI and carAB genes, and the Bacillus subtilis rocF gene increases the production of arginine and urea by the E. coli K-12 strain. The urea cycle in the bacteria is completed by the addition of arginase. The synthesis of carbamyl phosphate is another rate limiting step. The optimisation of level of overexpression of carbamyl phosphate synthetase, ornithine transcarbamylase and arginase removes byproducts from the medium. This increases the efficiency of ammonia assimilation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments
Article Abstract:
Microscopic studies reveal that Escherichia coli are present in stream water at higher densities, and the green fluorescent protein (GFP) gene fails to affect the fitness of the host strain. The use of GFP gene enables analysis of genetically engineered microorganisms (GEMs) by both plating and microscopy. CFU fails to show the presence of E. coli in stream water at high densities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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