Advances in the production of human therapeutic proteins in yeasts and filamentous fungi
Article Abstract:
The challenges faced while producing the therapeutic glycoproteins and the current state of development of engineered yeast strains that are able to produce humanized glycoproteins are discussed. Yeast and fungal protein expression systems are used for the production of many industrially relevant enzymes and are extensively used to produce proteins that are not actively expressed in Escherichia coli or require glycosylation for proper folding and biological activity.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Temperature-sensitive control of protein activity by conditionally splicing inteins
Article Abstract:
A system is created which forms temperature sensitive (TS) alleles depending on the conditionally vigorous inteins. If the minimum host requirements are needed for temperature sensitive intein splicing, then this method has the capacity to create and use the conditionally vigorous inteins in various host proteins and model systems that increases the utility of temperature sensitive alleles for evaluating the functions of protein.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Enhancement of therapeutic protein in vivo activities through glycoengineering
Article Abstract:
Glycosylation analogs with new N-linked glycosylation consensus sequence introduced into the protein are screened for the presence of additional N-linked carbohydrates and retention of in vitro activity. With the help of glycoengineering the activity of proteins are prolonged, thus allowing reduced frequency of administration.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Bank differences in the coordination of regulatory capital, earnings, and taxes. Foreign tax credit limitations and preferred stock issuances
- Abstracts: The perception of spatial layout in real and virtual worlds. Transfer of training from virtual reality
- Abstracts: Harnessing the power of database marketing. The buy-in benchmark: how staff understanding and commitment impact brand and business performance
- Abstracts: Imaging the pharmacodynamics of HER2 degradation in response to Hsp90 inhibitors. Serial in vivo imaging of the targeted migration of human HSV-TK-transduced antigen-specific lymphocytes