Crystalline protein domains and lipid bilayer vesicle shape transformations
Article Abstract:
A combination of confocal, fluorescence and differential interference contrast microscopy is used to study the effect of tethered streptavidin protein crystals on the bilayer curvature of several giant lipid unilamellar vesicles (GUVs). The analysis of the mechanism for the protein-mediated stabilization of cellular membranes prove that the crystalline streptavidin domains have a strong stabilizing effect on the lipid membranes, leading to a better and facilitated membrane budding.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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Liquid-liquid phase separations in urate oxidase/PEG mixtures: characterization and implications for protein crystallization
Article Abstract:
The experimental phase diagram of urate oxidase in the case of poly ethylene glycol (PEG) is characterized by determining the solubility curve and the dilute part of the liquid-liquid phase separation. The study shows that urate oxidase is a good model to study protein/PEG mixtures in the general context of protein crystallization.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
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Two-dimensional crystallization of streptavidin mutants
Article Abstract:
Research is presented to demonstrate how certain intermolecular interactions can be selectively changed to produce microscopic and macroscopic changes in two-dimensional crystalline properties.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1999
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